Resolution of Glycidyl Butyrate with Two Mixed and Screened Alkaline Lipase

Two mixed strains of microorganism capable of optically resolving racemic glycidyl butyrate have been isolated from soil and identified to be alkaline lipase,which one strain of lipase has no ideal result from tens of lipase.The suitable carbon source of the condition was starch and the nitrogen source was beaf extract.The proper temperature and initial pH for enzme formation were 30 ℃ and pH=8.0 respectively.Rotating rate was 200 r/min.Under two days fermentation,it can reach 1.5 u/mL.The technology of the two alkaline lipase in the optical resolution of glycidyl butyrate was studied.The condition was pH 6.0,28 ℃,200 r/min,0.50 g substrate added in the alkaline lipase liquid,when the glycidyl butyrate was transformed by 83.2%,the optical purity of(R)-glycidyl butyrate was 92.8% ee(enantiomeric excess).