Production of glycidyl butyrate hydrolase by Schizosaccharomyces

Abstrcat A high-yielding strain for producing glycidyl butyrate hydrolase was screened from soil. The conditions for enzyme formation of the strain were studied. The suitable carbon source of the medium was starch and the suitable nitrogen source was beaf extract. The optimum temperature and initial pH for enzyme formation were 30 ℃ and pH=8.0 respectively. Rotating speed was 200 r/min. In this condition, through 3days fermentation, it can obtained 1.17 U/mL. When the racemic substrate was enzymatically hydrolyzed to 50.5%, the optical purity of recovered (R)-ester was 81.4% ee(enantiomeric excess).