2012•Journal of Zhejiang University(Science Edition)Requires access
Microwave radiation-induced genetic damage of root-tip cells in Vicia faba
Zhu Mu-yuan
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Abstract
Microwave radiation-induced genetic damage was investigated in root-tip cells of Vicia faba.Taking the microwave as a mutagen in micronucleus and chromosome aberration assays,mitotic index,micronucleus frequency,and chromosome aberration frequency were detected in time-course treatments of Vicia faba root tip cells.The results showed that micronucleus frequencies,chromosome aberration frequencies in 6 microwave treatment groups were significantly higher than the controlling without microwave treatment(P0.001),while their mitotic index was much lower than that of control group(P0.001).During microwave treatments,micronucleus frequency and chromosome aberration frequency were increased in treatment time-dependent manner in root-tip cells,whereas the mitotic index was decreased.These data suggest that microwave causes obvious genetic damage to root-tip cells in Vicia faba.