Ultrasonic Extraction Process of Saponins from Polygonatum sibiricun

A comparative investigation was carried out on the extraction of saponins from Polygonatum sibiricun with three methods: hot water bath refluxing,ultrasonic wave aided extraction,and microwave aided extraction.Ultrasonic wave aided extraction was selected as an optimal method with the advantages of efficiency,safty,low cost,and non-damage of saponins.Using the content of ginsenoside Rb1 as an indictor,the optimal ultrasonic wave aided extraction conditions were determined as: solvent: 80 % ethanol,times of extraction: 2,temperature: 60 ℃,time of extraction: 50 minutes,and the ratio of material to solvent: 1∶15.Under the optimal conditions,the content of saponis in the extract was over 3.4%.Extraction temperature exhibited significant influence on the extraction,and time and the ratio did not show significant influence on the extraction.