Changes of CD4~+CD_(25)~+ and CD8~+CD_(28)~-Regulatory T Cells Levels in Peripheral Blood of Children with Asthma and Their Clinical Significances

Objective To compare the variation of CD4+CD25+ and CD8+CD28-regulatory T cell in childhood asthma,further to investigate the pathogenesis of childhood asthma and explore a new way for the early prevention and treatment so as to reduce its incidence.Met-hods The percentages of CD4+CD25+ and CD8+CD28-regulatory T cells in peripheral blood were detected by flowcytometry in 31 cases of childhood asthma who were in acute and alleviated period,and 23 cases of healthy children were enrolled as healthy control group.SPSS 13.0 software was used to analyze the data.Results The levels of CD4+CD25+ and CD8+CD28-regulatory T cells in peripheral blood of acute asthma(3.92%±1.75%;10.95%±3.46%) were significantly lower than those of healthy children(Pa0.01);the levels of CD4+CD25+ and CD8+CD28-regulatory T cells in peripheral blood of alleviated asthma (5.39%±2.01%;14.37%±3.92%) were significantly higher than acute childhood asthma(Pa0.05),but they were lower than those of healthy children (6.94%±2.27%;16.07%±4.28%)(Pa0.05).CD4+CD25+ and CD8+CD28-regulatory T cells were positively correlated with the course of childhood asthma (r=0.462,0.459 Pa0.001).Conclusions The regulatory abnormalities of immunologic network of CD4+CD25+ and CD8+CD28-regulatory T cells may probably lead to the onset and the development of children asthma.The levels of CD4+CD25+ and CD8+CD28-regulatory T cells can reflect the inflammation in the air tube,and are correlated with the course of the disease.Inducing the development of regulatory T cells can act as an effective method to treat childhood asthma,and it is expected to be a new way for treatment of childhood asthma.