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2012•Journal of Fujian University of Traditional Chinese MedicineRequires access

Establishment of Determination Method of Total Flavonoids in Portolaca oleracea L.

Zhang Li-ping

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Abstract

Objective To establish a method for determination of total flavonoids in Portolaca oleracea L.Methods The content of total flavonoids was detected by using UV spectrophotometry and rutin is used as reference substance.Results The linear range of total flavonoids was 0.004～0.064 mg·mL-1(r= 0.9999).Reference substance and sample had good stability and repeatability(RSD=0.001%,n=6) in 25 mins when the colour-display system had responded completely.The precision was high(RSD=0.0001%,n=6) and the average recovery was 100.8%(RSD=2.66%,n=6).Conclusion The method is simple and accurate,and can be used effectively for the quality control of Portolaca oleracea L..

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Objective To establish a method for determination of total flavonoids in Portolaca oleracea L.Methods The content of total flavonoids was detected by using UV spectrophotometry and rutin is used as reference substance.Results The linear range of total flavonoids was 0.004～0.064 mg·mL-1(r= 0.9999).Reference substance and sample had good stability and repeatability(RSD=0.001%,n=6) in 25 mins when the colour-display system had responded completely.The precision was high(RSD=0.0001%,n=6) and the average recovery was 100.8%(RSD=2.66%,n=6).Conclusion The method is simple and accurate,and can be used effectively for the quality control of Portolaca oleracea L..

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Available abstract

Objective To establish a method for determination of total flavonoids in Portolaca oleracea L.Methods The content of total flavonoids was detected by using UV spectrophotometry and rutin is used as reference substance.Results The linear range of total flavonoids was 0.004～0.064 mg·mL-1(r= 0.9999).Reference substance and sample had good stability and repeatability(RSD=0.001%,n=6) in 25 mins when the colour-display system had responded completely.The precision was high(RSD=0.0001%,n=6) and the average recovery was 100.8%(RSD=2.66%,n=6).Conclusion The method is simple and accurate,and can be used effectively for the quality control of Portolaca oleracea L..

Key concepts: Rutin, Repeatability, Chromatography, Chemistry, Linear range, Linear relationship, Mathematics, Detection limit

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