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Study on Determination of Total Flavonoids in Bee-Pollen

Jie Nan Dong

Abstract

Flavonoids are widely studied in natural products because of their multifarious bioactivities to human body. The paper compared tree methods of determination and confirmed a method for quantitative determination of total flavonoids in bee- pollen by ployamide-UV spectrophotometry. The standard sample was rutin. The optimum determination wavelength was 360nm. The results showed that a good linear relationship (R2=0.9998) between OD and the concentration of analyte is obtained, within the range of 0.005～0.025mg/ml. The average recovery rate is 95.53%. The stability, precision and repetition are very good, with RSD 2.124%(n=3), 0.488%(n=3), 0.875%(n=5) respectively. The content of the total flavonoids in rape pollen is 1.54% by ployamide-UV spectrophotometry.

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Key concepts: Rutin, Bee pollen, Chemistry, Analyte, Chromatography, Spectrophotometry, Pollen, Linear relationship