Study on Impact Factors of Manganese-Dependent Peroxidase Produced by White Rot Fungi

Taking enzyme activity of manganese-dependent peroxidase as the assessment index,the effects of several factors on the characterizations of growth and production of manganese-dependent peroxidase(MnP)by white rot fungi(Phanerochaete chrysosporium)were studied.Results indicated that,veratryl alcohol(150mg·L-1),benzyl alcohol(150mg·L-1)and Tween 80(14mg·L-1)could stimulate the growth of white rot fungi and activity of MnP.Effects on the growth of white rot fungi and activity of MnP were the same with addition of Ca2+and Fe2+,which was promoted at beginning and inhibited later.When Cu2+concentration was lower than0.4mg·L-1,Cu2+cound promote the growth of white rot fungi and activity of MnP,while it could inhibit the growth of white rot fungi and activity of MnP when the concentration was mare than 0.4mg·L-1.