Determination of isoprothiolane residues in aquatic products by gas chromatography

A gas chromatographic method was developed for the determination of isoprothiolane residues in aquatic products.The isoprothiolane residue in the test sample are extracted with n-hexane-acetone(2+1,v/v).After the extracts is evaporated,the residues is cleaned up by solid phase extraction(SPE)with a neutral aluminum oxide cartridge and a florisil cartridge.The residue is determined by GC with ECD-detector,using external standard method.The liner range was 0.0025～0.25μg·mL-1 for isoprothiolane.The correlation coefficients were 0.99960 for isoprothiolane.The regression equation was y= 57194.44 x-71.82.The average recoveries of isoprothiolane in spiked samples ranged from 84.2%～08.7%,and the relative standard deviations were between 3.94%～6.20%.The limit of detection was 0.005mg·kg-1 in method.