Molecular Cloning and Activity Analysis of a Promoter of an Arabidopsis thaliana Gene At2g04450

5′-UTR of At2g04450 gene was cloned by PCR from Arabidopsis thaliana.To investigate the tissue expression pattern and the possible response to pathogen infection of the cloned regulatory sequence,an expression vector containing this sequence fused with GUS was constructed for transformation into A.thaliana by using agrobacterium-mediated method.Histochemical staining of transgenic A.thaliana showed that GUS reporter gene was predominantly expressed in roots,and the activity of the promoter can not be induced by the PstDC3000.This research will facilitate the study of At2g04450 gene function.