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THE OPTIMUM METHODS OF EXTRACTION AND PURIFICATION OF POLYSACCHARIDE FROM PLEUROTUS FERULAE LENZI MYCELIUM

Long Yu

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Abstract

This research showed a high yield Pleurotus ferulae Lenzi strain by low energy ion implantation that extraction methods of polysaccharide from mycelium were studied by orthogonal experiments, rotation and combinational design of quadric regression the basis of determination of the extraction rates of polysaccharides.The extracting conditions, ethanol precipitation, saline concentration, remove protein,H2O2 discolor had been discussed. The results showed that the optimal condition are 15 times water ,100 ℃,3 h,extraction of polysaccharides 3 times, precipitate condition is Ammonium sulfate with ethanol(Ammonium sulfate and polysaccharide solution is 1:5(m/m), ethanol and polysaccharide solution is 1:1 (v/v) )8 h, H2O2 discolor 6 h.

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What this paper is about

This research showed a high yield Pleurotus ferulae Lenzi strain by low energy ion implantation that extraction methods of polysaccharide from mycelium were studied by orthogonal experiments, rotation and combinational design of quadric regression the basis of determination of the extraction rates of polysaccharides.The extracting conditions, ethanol precipitation, saline concentration, remove protein,H2O2 discolor had been discussed. The results showed that the optimal condition are 15 times water ,100 ℃,3 h,extraction of polysaccharides 3 times, precipitate condition is Ammonium sulfate with ethanol(Ammonium sulfate and polysaccharide solution is 1:5(m/m), ethanol and polysaccharide solution is 1:1 (v/v) )8 h, H2O2 discolor 6 h.

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Available abstract

This research showed a high yield Pleurotus ferulae Lenzi strain by low energy ion implantation that extraction methods of polysaccharide from mycelium were studied by orthogonal experiments, rotation and combinational design of quadric regression the basis of determination of the extraction rates of polysaccharides.The extracting conditions, ethanol precipitation, saline concentration, remove protein,H2O2 discolor had been discussed. The results showed that the optimal condition are 15 times water ,100 ℃,3 h,extraction of polysaccharides 3 times, precipitate condition is Ammonium sulfate with ethanol(Ammonium sulfate and polysaccharide solution is 1:5(m/m), ethanol and polysaccharide solution is 1:1 (v/v) )8 h, H2O2 discolor 6 h.

Key concepts: Polysaccharide, Mycelium, Extraction (chemistry), Ethanol precipitation, Chemistry, Ammonium sulfate, Chromatography, Pleurotus

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THE OPTIMUM METHODS OF EXTRACTION AND PURIFICATION OF POLYSACCHARIDE FROM PLEUROTUS FERULAE LENZI MYCELIUM — Research Paper | ScholarLens