Study on the Deproteinization of Corn Stigma Polysaccharide by Sevag Method

[Objective] The research aimed to optimize the deproteinization process conditions of polysaccharides of corn stigma by sevag method.[Method]On the basis of single factor experiment,the deproteinization process was tested by orthogonal experiment of 4 factors and 3 levels.4 factors were volume proportion of n-butanol and chloroform,volume ratio of polysaccharide liquid and sevag reagent,shaking time and times of deproteinization.[Result]The optimal process of deproteinization was as follows:the volume proportion of n-butanol and chloroform was 5:1,sample liquid and sevag reagent ratio was 2∶ 1 and shaking time was 8 min,deproteinization times was 4 times.[Conclusion] Under the best conditions,the rate of deproteinization was 64.2% and the loss of polysaccharide was 34.3% .Polysaccharide purity increased from 11.5% to 32.3% .