Methods of Deproteinization and Decoloration for Fucoidan

Using Sevag method,trichloroacetic acid(TCA) method and combined method of Sevag and TCA to remove protein from fucoidan,and impacts of deproteinization and non-deproteinization on decoloration of fucoidan were studied.The results showed that the combined method of Sevag and TCA had the best effect of deproteinization;when operating Sevag method twice and adding 1.3 mL/10mL of TCA to polysaccharide solution,the removal rate of protein was 78.68%,the loss rate of polysaccharide was 28.89%,the decolorization rate of polysaccharide stock solution was 29.13%,and the decolorization rate of polysaccharide deproteinization solution was 85.17%.