Tissue Culture in Yucca gloriosa Linnaeus

Using the stem tip and tender stem shoot and leaf as the explants, the tissue culture of Yucca gloriosa Linnaeus was studied. The callus-inducing rate from the tender leaf was the highest and the induced calli from the tender stem were the best for subculture, while the induced calli from the stem tip produced best adventitious buds. The optimum media for each culture stages were: MS + 6-BA 3.0 mg/L + NAA 0.2 mg/L + sucrose 30 g/L for formation of callus; MS + 6-BA 8.0 mg/L + NAA 0.1 mg/L + 30 g/L for callus subculture for multiplication; MS + 6-BA 5.0 mg/L + KT 1.0 mg/L + sucrose 30 g/L for inducing adventitious buds; MS + 6-BA 4.0 mg/L + NAA 0.05 mg/L + CW 100 mL/L+ sucrose 30 g/L for buds multiplication; 1/2 MS + NAA 0.2 mg/L + sucrose 30 g/L for rooting.