Effect of NAA and 6-BA on Wild Dioscorea alata Callus Induction and Adventitious Bud Differentiation and Rooting

The effects of 6-BA and NAA on callus induction,adventitious bud differentiation and rooting of wild Dioscorea alata Linn. were exzamined. The results showed that the best medium for callus induction was MS+1.0 mg / L 6-BA+0.2 mg / L NAA+30 g / L sucrose+ 6.0 g / L agar. The best medium for buds differentation was MS+0.5 mg / L 6-BA+0.1 mg / L NAA+30 g / L sucrose + 6.0 g / L agar. The best rooting medium was MS+0.01 mg / L 6-BA+0.3 mg / L NAA+30 g / L sucrose+6.0 g / L agar. The survival rate of transplanting was over 95% after exercising seedling in ambient temperature for 7~10 days and off closure for2~3 days.