2011•Northern HorticultureRequires access
Study on Tissue Culture of Euphorbia pulcherrima
Xiaoli Wang
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Abstract
Axillary buds,apical buds,stems and leaves of Euphorbia pulcherrima were used as explant,tissue culture of Euphorbia pulcherrima way and establishment system of plant regeneration were studied.The results showed that the best explants were axillary buds,buds and stems.The best explants of callus was stems,the most favorable media was MS+6-BA 0.5 mg/L+2,4-D 2.0 mg/L+sucrose 30 g/L.The best explants of induced buds was axillary buds,apical buds and callus,the most favorable media of apical bud and axillary bud as explant:MS+6-BA 0.6 mg/L+NAA 0.1 mg/L+sucrose 30 g/L;The most favorable media of callus as explant:MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+sucrose 30 g/L.The subculture media was MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+sucrose 30 g/L.The rooting media 1/2MS+NAA 1.0 mg/L+sucrose 30 g/L.