The effect of MIP-1α combined IL-8 on mouse CD34~+ cells′ proliferation

Objective To study the effect of MIP 1αcombined IL 8 in different concentration on mouse CD34 + cell proliferation. Methods Mouse CD34 + cells were gotten from mouse femur bone marrow cells by MiniMACS, and then these CD34 + cells were cultivated in liquid system and 0.9% methyl cellulose system with different concentration of MIP 1αand IL 8 in different group. In order to know how about the proliferation of these CD34 + cells, the alive cell number, the clone formation units of CFU GM, CFU E, CFU Mix and the cell cycle were measured. Results (1) The alive cell number of 10ng/ml and 50ng/ml group of liquid system was significantly less than that of 1ng/ml and blank group ( P 0.01), but there was no difference between 10ng/ml and 50ng/ml group, so do that between 1ng/ml and blank group. The clone formation units experiment showed the same result as liquid system culture. (2) The G 0/G 1 cells of 10ng/ml and 50ng/ml group were significantly more than that of 1ng/ml and blank group, although there was no any difference between 10ng/ml and 50ng/ml group, also between 1ng/ml and blank group. Conclusion MIP 1α combined IL 8 in certain concentration can inhibit the proliferation of mouse CD34 + cells.