The effect of low dose MIP-1α combined IL-8 on cord blood CD34~+ cells′ clone formation

Objective To study the effect of low dose MIP 1α combined IL 8 on cord blood cells' clone formation.Method Cord blood MNC were isolated from other cells by centrifugation in medium of Ficoll Hypagque (1.007).After that,CD34 + cells were got from these MNC buy MiniMACS,and then these CD34 + cells were divided into five groups : control group,10ng/ml MIP 1α combined 10ng/ml IL 8 group,1ng/ml MIP 1α combined 1ng/ml IL 8 group,20ng/ml MIP 1αgroup and 20ng/ml IL 8 group.Then these cells were cultivated in 0.9% methylcellulose system.Several days later,count the clones of CFU GM,CFU E and CFU Mix of each group.Result At the concentration of 1ng/ml MIP 1α combined IL 8 can not inhibit the clone formation obviously,but 10ng/ml MIP 1α combined IL 8 in same concentration can inhibit the clone formation obviously.Neither MIP 1α nor IL 8 can inhibit the clone formation if they were used alone in our study.Conclusion 10ng/ml MIP 1α combined IL 8 can synergies each other to inhibit cord blood cells,clone formation.