Effect of low dose MIP-1α combined IL-8 on clone formation of hurman lone marrow hematopoietic cells

Objective To study the effect of low dose Macrophage inflamatory prolein α (MIP 1α) combined with IL 8 on clone formation of human bone marrow hematopoietic cells. Methods Bone marrow hematopoietic cells were divided into 5 groups: control group (with no MIP α or IL 8) , 10 mg/ml combination group (with 10 ng/ml MIP lx and 10 ng/ml IL 8), 1 mg/ml combination group (with 1 ng/ml MIP lα and 1 ng/ml IL 8) ,MIP lα group (with 20 ng/ml MIP), and IL 8 group (with 20 ng/ml IL 8). Then these cells were cultivated in 0 9% methyl cellulose system. Several days later, count the clones of CFU GM, CFU E and CFU Mix of each group. Results At the concentration of 1 ng/ml, combination of MIP 1α and IL 8 didn't inhibit the clone formation but the 10 ng/ml combination group did obviously. Neither MIP 1α nor IL 8 alone did it in our study. Conclusion Combination of MIP 1α and IL 8 both at 10 ng/ml can coordinate each other to inhibit the clone formation of human bone marrow hematopoietic cells.