The diagnostic Value of Three Methods Among autoimmune diseases

Objective To study more significant detective combination by comparing three methods of antinuclear antibody, extractable nuclear antigen and antinuclear antibodies 3.Methods Three methods were adopted as follows:detecting antinuclear antibody with HEP-2 and prime hepar as antigen media,determining extractable nuclear antigen by EUROASSAY including nRNP/Sm、Sm、SSA、SSB、Scl-70 and Jo-1,using crithidia luciliae as antigen,to detect ds-DNA antibody with indirect immunofluorescence assay,and to study 15 autoantibodies by EUROLINE.Results To compare positive rates of three dilution(1:10, 1:80,1:100) in ANA detection,there existed significant difference(P0.01) between two dilution of 1:10 and 1.80 only,.In the detection of multiple nuclear types(cytoplasmic pattern,centromere pattern and homegeneous pattern etc),the positive rate of antinucleatr antibodies 3 was higer than that of ENA.IIF was prior to the EUROLINE in the detection of ds-DNA.Conclusion During detecting ANA with IIF,the dilution of 1:80 has advantages in raising positive rate,and ANA3 of 15 antigens provides facilities for clinical diagnosis.