ScholarLens Back to search
2004•China Public HealthRequires access

Effects of fluoride on DNA damage and apoptosis in human embryo hepatocytes

Tao Xia

Open publisher page 0 citations

Abstract

Objective To explore the effects of fluoride on DNA damage and apoptosis in human embryo hepatocytes.Methods DNA damage and apoptosis were measured after human embryo hepatocytes were incubated with 40,80,and 160 μg/ml sodium fluoride using Single Cell Electrophosis (SCGE) and Flow Cytometry (FCM),respectively.Results The DNA damage of human embryo hepatocytes in the fluoride gruoup was significantly higher than the control group.Ridit value were 0.582,0.815 and 0.892 respectively.The percentage of apoptosis in the middle and high fluoride group were markedly higher than the control group.Morevoer,the rate of DNA damage and the percentage of apoptosis in high fluoride group were significantly higher than the low fluoride group.Conclusion Fluoride exposure can cause DNA damage and apoptosis in human embryo hepatocytes, and there existed dose-effect relationship to some extent.

About this research paper

What this paper is about

Objective To explore the effects of fluoride on DNA damage and apoptosis in human embryo hepatocytes.Methods DNA damage and apoptosis were measured after human embryo hepatocytes were incubated with 40,80,and 160 μg/ml sodium fluoride using Single Cell Electrophosis (SCGE) and Flow Cytometry (FCM),respectively.Results The DNA damage of human embryo hepatocytes in the fluoride gruoup was significantly higher than the control group.Ridit value were 0.582,0.815 and 0.892 respectively.The percentage of apoptosis in the middle and high fluoride group were markedly higher than the control group.Morevoer,the rate of DNA damage and the percentage of apoptosis in high fluoride group were significantly higher than the low fluoride group.Conclusion Fluoride exposure can cause DNA damage and apoptosis in human embryo hepatocytes, and there existed dose-effect relationship to some extent.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To explore the effects of fluoride on DNA damage and apoptosis in human embryo hepatocytes.Methods DNA damage and apoptosis were measured after human embryo hepatocytes were incubated with 40,80,and 160 μg/ml sodium fluoride using Single Cell Electrophosis (SCGE) and Flow Cytometry (FCM),respectively.Results The DNA damage of human embryo hepatocytes in the fluoride gruoup was significantly higher than the control group.Ridit value were 0.582,0.815 and 0.892 respectively.The percentage of apoptosis in the middle and high fluoride group were markedly higher than the control group.Morevoer,the rate of DNA damage and the percentage of apoptosis in high fluoride group were significantly higher than the low fluoride group.Conclusion Fluoride exposure can cause DNA damage and apoptosis in human embryo hepatocytes, and there existed dose-effect relationship to some extent.

Key concepts: Fluoride, Apoptosis, Embryo, DNA damage, Sodium fluoride, Flow cytometry, DNA, Chemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effects of fluoride on DNA damage and apoptosis in human embryo hepatocytes — Research Paper | ScholarLens