2001•Endemic Diseases BulletinRequires access
Construction and Identification of Eukaryotic Expression Plasmid of Murine IL-3
Zhenghai Ma
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Abstract
The murine interleukin-3(IL-3) is an important immunoregulatory factor that can be used as the immunological adjuvant in mouse model. In this paper, the murine IL-3 gene is cloned into eukaryotic expression plasmid pCDNA 3 exactly by identification of recombinant and sequencing. The positive clone named as pCDNA 3IL-3. This plasmid was transformed into E.coli which was cultured overnight, then the plasmid was substracted as well as purificated largely, and the purification and concentration was examined with spectraphotometer.