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2001•Unpublished venueRequires access

Construction and Identification of Eukarytic Express Plasmid of Murine IL-2

Zhong Zhe

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Abstract

The murine interleukin-2(IL-2) is an important immunoregulatory factor that can be used as the immunological adjuvant in mouse model.The murine IL-2 gene has been cloned into eukaryotic express plasmid pCDNA\-3 after identification of recombinant and sequencing.The positive colne is named pCDNA\-3LI-2.This plasmid was turned into E.coll.which was cultured overnight.Then the plasmid was substracted and purificated and the purification and concentration were examined with spectraphctometer.

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What this paper is about

The murine interleukin-2(IL-2) is an important immunoregulatory factor that can be used as the immunological adjuvant in mouse model.The murine IL-2 gene has been cloned into eukaryotic express plasmid pCDNA\-3 after identification of recombinant and sequencing.The positive colne is named pCDNA\-3LI-2.This plasmid was turned into E.coll.which was cultured overnight.Then the plasmid was substracted and purificated and the purification and concentration were examined with spectraphctometer.

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Available abstract

The murine interleukin-2(IL-2) is an important immunoregulatory factor that can be used as the immunological adjuvant in mouse model.The murine IL-2 gene has been cloned into eukaryotic express plasmid pCDNA\-3 after identification of recombinant and sequencing.The positive colne is named pCDNA\-3LI-2.This plasmid was turned into E.coll.which was cultured overnight.Then the plasmid was substracted and purificated and the purification and concentration were examined with spectraphctometer.

Key concepts: Plasmid, Recombinant DNA, Biology, Gene, Molecular biology, Plasmid preparation, Microbiology, Genetics

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