Construction and expression of recombinant mouse interleukin 4 eukaryotic expressing vector

Objective To construct an eukaryotic expression plasmid contained murine IL-4 gene and investigate the expression of the recombinant plasmid in 293T cells. Methods The gene of murine IL-4 was obtained by RT-PCR and then was cloned into an eukaryotic expression plasmid pcDNA3. 0,and then the recombinant plasmid was confirmed by electrophoresis and DNA sequencing. The plasmid was transfected into 293T cells by liposome transfecting technique and was identified by ELISA. Results The recombinant plasmid pcDNA3. 0-mIL-4 had been constructed correctly.Meanwhile,the supernatant collecting from 293T cells,transfected with the plasmid was showed reactivity with antibody against IL-4. Conclusion The recombinant pcDNA3. 0-mIL-4 was constructed successfully and highly expressed in293T cells,which will become the basis for the further study of the biologic activity of IL-4 in vivo.