Application Analysis of Quantitative PCR detection of Mycoplasma pneumoniae Mycoplasma pneumoniae pneumonia

Objective To investigate the quantitative PCR in the diagnosis of mycoplasma pneumonia feasibility and superiority. Method 186 cases of patients with pneumonia were treated with MP rapid culture method and the real-time PCR testing respectively. the sensitivity and specificity of two methods data were compared. Results The diagnosis of mycoplasma pneumonia sensitivity,positive predictive value,false negative rate and diagnostic accuracy of rapid culture method and real-time PCR had no significant difference( P 0. 05); mycoplasma pneumonia diagnosis specificity of Real-time PCR was 85. 5%( 65 /76),significantly higher than 77. 6% of rapid culture( 59 /76),the false positive rate was 14. 5%( 11 /76),significantly lower than the 22. 4% of rapid culture( 17 /76),the difference was statistically significant( P 0. 05). Conclusion The rapid culture method and real-time PCR diagnosis of mycoplasma pneumonia sensitivity have no different,but the specificity of real-time PCR method is superior to rapid culture method. The clinical application of the principles of these two different combinations can improve the detection rate of Mycoplasma pneumoniae infection,while also supporting each other,to reduce experimental error and improve the accuracy of detection.