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2011•Qilu Pharmaceutical AffairsRequires access

Determination of puerarin in Waiganfenghan Granules by HPLC

Fan Wang

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Abstract

Objective To determine the content of puerarin in Waiganfenghan Granules by RP-HPLC.Methods The determination was carried out by Kromasil C18 column.The mobile phase consisted of acetonitrile-water-menthol(8:86:6).The flow rate was 1 mL·min-1 and the detection wavelength was 250 nm.Results The linear range of puerarin was 50～100 μg·mL-1(r=0.999 6) and the average recovery was 97.3%,with the RSD was 1.13%(n=6).Conclution The method was simple,sensitive,accurate and can be used for the determination of puerarin in Waiganfenghan Granules.

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What this paper is about

Objective To determine the content of puerarin in Waiganfenghan Granules by RP-HPLC.Methods The determination was carried out by Kromasil C18 column.The mobile phase consisted of acetonitrile-water-menthol(8:86:6).The flow rate was 1 mL·min-1 and the detection wavelength was 250 nm.Results The linear range of puerarin was 50～100 μg·mL-1(r=0.999 6) and the average recovery was 97.3%,with the RSD was 1.13%(n=6).Conclution The method was simple,sensitive,accurate and can be used for the determination of puerarin in Waiganfenghan Granules.

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Available abstract

Objective To determine the content of puerarin in Waiganfenghan Granules by RP-HPLC.Methods The determination was carried out by Kromasil C18 column.The mobile phase consisted of acetonitrile-water-menthol(8:86:6).The flow rate was 1 mL·min-1 and the detection wavelength was 250 nm.Results The linear range of puerarin was 50～100 μg·mL-1(r=0.999 6) and the average recovery was 97.3%,with the RSD was 1.13%(n=6).Conclution The method was simple,sensitive,accurate and can be used for the determination of puerarin in Waiganfenghan Granules.

Key concepts: Puerarin, Chromatography, Chemistry, High-performance liquid chromatography, Linear range, Linear relationship, Detection limit, Mathematics

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