Analysis of immunophenotype in 93 cases of acute leukemia

Objective To explore the significance of immunophenotype by flow cytometry in the diagnosis and treatment of acute leukemia(AL).Methods Flow cytometry and CD45/side scatter(SSC) gating were used to analyze antigenic expressions in 93 patients with AL.Results The antigens expressed in 69 patients with acute myeloid leukemia(AML) were mainly CD13,CD33,HLA-DR and CD117;lymphoid antigens expression was identified in 20.2% of AML patient,there was a significant difference between CD7+ and CD7-in the percent CR(P0.05).In 13 patients with acute B-lymphoblastic leukemia,the expressed antigens were mainly CD19,CD34,HLA-DR,CD20,CD22,and 31% of these patients were found to be positive for myeloid antigen expression.There was no significant difference between CD13+ and CD13-in the percent CR(P0.05).The antigens expressed in 7 patients with acute T-lymphoblastic leukemia were mainly CD5,CD3,CD7,and CD34.Conclusion Immunological classification by flow cytometry has important value in therapy and prognosis of AL.