Determination of the Concentration of Baicalin and Geniposide in Qingjin Huatantang by HPLC

Objective:To establish the method for determination of baicalin and geniposide in the Qingjin Huatantang.Method:High performance liquid chromatography was applied on a Agilent C18(4.6 mm × 250 mm,5μm) column at temperature 30 ℃,the mobile phase for baicalin consisted of methanol and 0.4% phosphoric acid(47:53) at detection wavelength of 280 nm,the flow rate was 0.8 mL.min-1;the mobile phase for geniposide consisted of methanol and water(25:75) at detection wavelength of 238 nm,the flow rate was 1 mL.min-1.Results:The content of baicalin in Qingjinhuatantang was 221.59 mg /dose,the content of geniposide was 335.73mg /dose.The mean recovery of baicalin and geniposide was 97.97 % and 98.36 % respectively.Conclusion:This method is simple,rapid,accurate and stable,as well as reproducible and low cost.