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2007•ZhongchengyaoRequires access

Determination of geniposide and baicalin in four preparations by HPLC

Wang Qiang

Open publisher page 1 citations

Abstract

AIM:To established a method for determining geniposide and baicalin in four preparations cantaining Fructus gardeniae and Radix Scutellariae by HPLC.METHODS:The separation was performed on Lichrospher C_ 18 column;using water-phosphoric acid(53∶0.2)and acetonitrile as a mobile phase,gradient elution.The detection wavelength was 240 nm.The flow rate was at 1.0 mL/min.The column temperature was at room temperature.RESULTS:The linear range of geniposide was in the range of 1.9-76 μg/mL,r=0.999 9,the average recovery was 101.3%.The linear range of baicalin was 10.58-423 μg/mL,r=0.999 9,the average recovery was 100.6%.CONCLUSION:This method is rapid,sensitive and accurate.It indicates that geniposide and bai-calin in traditional Chinese medicine preparations can be determinated simultaneously,and the method can be used for quality control.

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AIM:To established a method for determining geniposide and baicalin in four preparations cantaining Fructus gardeniae and Radix Scutellariae by HPLC.METHODS:The separation was performed on Lichrospher C_ 18 column;using water-phosphoric acid(53∶0.2)and acetonitrile as a mobile phase,gradient elution.The detection wavelength was 240 nm.The flow rate was at 1.0 mL/min.The column temperature was at room temperature.RESULTS:The linear range of geniposide was in the range of 1.9-76 μg/mL,r=0.999 9,the average recovery was 101.3%.The linear range of baicalin was 10.58-423 μg/mL,r=0.999 9,the average recovery was 100.6%.CONCLUSION:This method is rapid,sensitive and accurate.It indicates that geniposide and bai-calin in traditional Chinese medicine preparations can be determinated simultaneously,and the method can be used for quality control.

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Available abstract

AIM:To established a method for determining geniposide and baicalin in four preparations cantaining Fructus gardeniae and Radix Scutellariae by HPLC.METHODS:The separation was performed on Lichrospher C_ 18 column;using water-phosphoric acid(53∶0.2)and acetonitrile as a mobile phase,gradient elution.The detection wavelength was 240 nm.The flow rate was at 1.0 mL/min.The column temperature was at room temperature.RESULTS:The linear range of geniposide was in the range of 1.9-76 μg/mL,r=0.999 9,the average recovery was 101.3%.The linear range of baicalin was 10.58-423 μg/mL,r=0.999 9,the average recovery was 100.6%.CONCLUSION:This method is rapid,sensitive and accurate.It indicates that geniposide and bai-calin in traditional Chinese medicine preparations can be determinated simultaneously,and the method can be used for quality control.

Key concepts: Baicalin, Chromatography, Chemistry, Phosphoric acid, Gradient elution, High-performance liquid chromatography, Radix (gastropod), Linear range

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