Anti-tumor Effect of Phlomiol and Its Mechanism Study

OBJECTIVE:To study the anti-tumor effect of phlomiol,and its influences on the activity of telomerase and cells phases.METHODS:Serologic pharmacological method,in vivo and in vitro anti-tumor experiments were adopted in the study.The inhibition effect of serum containing phlomiol on the proliferation of K562 tumor cells in vitro were detected by MTT method.The activity of telomerase in K562 tumor cell was determined by TRAP-PCR-ELISA.Flow cytometry was used to analyze the change of cell cycle.In vivo experiment,mice was inoculated with hepatic cancer H22 cells and the mice were orally administrated with phlomiol for 14 days.The inhibition rate of tumor was caculated.RESULTS:Serum containing 10%～50% phlomiol inhibited the proliferation of K562 tumor cells in vitro in dose-dependent manner(P0.05).Phlomiol inhibited the activity of telomerase in K562 tumor cells.The inhibition effect was in dose-dependent manner(P0.01).Meanwhile the inhibition effect increased gradually when the action time extended in the same dose of phlomiol group(P0.05).The content of cell in K562 tumor cells were markedly increased in G2/M phase(P0.01).Phlomiol(2.5,5,10 mg·kg-1,i.g) inhibited the growth of implanted hepatic cancer H22 cells signiflcantly.CONCLUSION:The anti-tumor activity of phlomiol is performed by inhibiting the activity of telomerase in tumor cells.