Studies on Separation and Purification of Total Flavones from Gynostemma Pentaphyllum with Macroreticular Resin

Objective:To set up the technology for adsorbing and separating total flavone in Gynostemma pentaphyllum with macroporous adsorption resin,and D101 macroporous adsorption resin was invested.Methods:The static adsorption and separation were used in investigation of macroporous adsorption and separation;the dynamic adsorption and adsorption were used in studying the condition of adsorption and separation.The total flavone adsorption capacity and desorption rate were used as the evaluation criteria.The UV spectrophotometric method was used in the determination of total flavone content.Results:The results showed that the D101 was the best for adsorbing and separating the total flavone in Gynostemma pentaphyllum in the following technological conditions:the concentration of Gynostemma pentaphyllum sample extract was 8.95 mg/mL;the maximum adsorbing capacity for total flavone in Gynostemma pentaphyllum was 9.06 mg/mL;the current velocity was 6BV/h;the eluting reagent was 50% ethanol.Conclusion:It is the best way to use D101 resin to separate the total flavone in Gynostemma pentaphyllum under the technological conditions,the purification technology is better,the method of purifying total flavones is feasiable.