Effect of phosphatase of regenerating liver cell-1 on invasion of human lung cancer cell

Objective:To study the effects and mechanism of phosphatase of regenerating liver cell-1(PRL-1)small interfering RNA(siRNA)on invasion of human lung cancer cell.Methods: After lung cancer cell line A549 were transfected by PRL-1 siRNA,the mRNA and protein of PRL-1 were determined by real time RT-PCR and western blot assay,respectively.The anchorage-independent growth was exmined by clon formation in soft agar,and invasion ability was evaluated by boyden chamber model. Results: The siRNA could down-regulate the level of mRNA and protein of PRL-1 in a dose-and time-dependent manner.Suppression of of PRL-1 expression can inhibit invasion ability and anchorage-independent growth in dose-dependent manners(P0.05,P0.05). Conclusion: PRL-1 gene might play an important role in development of human lung cancer,and down-regulation by PRL-1 siRNA could inhibit invasion of human lung cancer cell.