Effects of phosphatase of regenerating liver cell-1 on invasion of human lung cancer cells

Objective To study the effects of phosphatase of regenerating liver cell-1 (PRL-1) small interfering RNA (siRNA) on invasion of lung cancer cells and the mechanism. Methods After lung cancer cell line A549 cells were transfected by PRL-1 siRNA, the mRNA and protein expression levels of PRL-1 and matrix metalloproteinase-2 and-9 were detected by real time reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, respectively. The anchorage-independent growth was examined using colon formation in soft agar, and invasion ability was evaluated by boyden chamber model. Results The anchorage-independent growth showed that the colonies were 17.8 ± 1.6, 13.6 ± 1.5, 8.8 ± 1.4, and 23.6 ±1.8 in different groups (3.125, 6.25, 12.5 nmol/L siRNA and control), respectively. The boyden chamber results revealed that the number of invasion cells was 33.6 ± 3.1, 19.5 ±1.9, 8.1 ±1.8 and 49.4 ±3.3 in different groups (3.125, 6.25, 13.5 nmol/L siRNA, and control) , respectively. The mRNA and protein expression levels of MMP-2,-7 and-9 in transfection group with PRL-1 siRNA were down-regulated as compared with control group in lung cancer cells. Conclusion PRL-1 siRNA can inhibit invasion through down-regulating MMPs of lung cancer cells. Key words: Lung carcinoma;  Invasion;  Matrix metalloproteinase