Silencing phosphatase of regenerating liver cell-3 gene regulates proliferation and apoptosis of LNCaP cells

Objective To study the effects of small interfering RNA (siRNA) silencing phosphatase of regenerating liver cell 3 (PRL-3) on proliferation and apoptosis of human prostate cancer LNCaP cells.Methods After prostate cancer LNCaP cells were transfected by PRL-3 siRNA,the mRNA and protein of PRL-3 were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting,respectively.The growth of LNCaP cells was exmined by cell counting kit-8 (CCK-8).Flow cytometry was used to detect apoptosis.Results The siRNA could downregulate the mRNA and protein expression level of PRL-3 in a dose-dependent manner (P ＜ 0.05).Low doses of PRL-3 siRNA did not affect the proliferation of LNCaP cells,but 100 nmol/L PRL-3 siRNA could effectively inhibit the proliferation of LNCaP cells after 48 h in vitro (P ＜ 0.05).Apoptosis rate of LNCaP cells with low,middle and high PRL3 siRNA interventions in vitro was (28.1 ± 3.8) ％,(25.2 ± 2.5) ％,and (27.1 ± 0.7) ％ respectively (P ＜ 0.05).Conclusion Downregulating PRL-3 could inhibit proliferation of LNCaP cells and promote apoptosis of LNCaP cells.PRL-3 gene might play an important role in proliferation and apoptosis of LNCaP cells. Key words: Prostate cancer;  Phosphatase of regenerating liver cell 3 ;  RNA interference