Role of mycoplasma infection and expression of Ki67 protein in the pathogenesis,development and prognosis of laryngeal carcinoma

OBJECTIVE Study the role of mycoplasma infection and expression of Ki67 protein in the pathogenesis, development and prognosis of laryngeal carcinoma. METHODS Immunohistochemistry method was used to study 145 specimens of laryngeal carcinoma tissues, 25 specimens of precarcinoma tissues, 31 specimens of vocal cord polyps and 15 specimens of normal tissues adjacent to laryngeal carcinoma. RESULTS ①The positive rates of PD4 and Ki67 were 45.52%(66/145) and 82.76 % (120/145) in laryngeal carcinoma tissue, 16.00 % (4/25) and 32.00 % (8/25) in precarcinoma tissue, 12.90 % (4/31) and 22.58 % (7/31) in vocal cords polyps, 6.67 % (1/15) and 0 (0/15) in normal tissues adjacent to laryngeal carcinoma. ②The positive rates of PD4 and Ki67 were higher in the advanced laryngeal carcinoma cases than that in the early laryngeal carcinoma cases. The positive rates of PD4 and Ki67 were higher in laryngeal carcinoma cases with cervical metastasis than that laryngeal carcinoma cases without cervical metastasis(P0.01). The PD4 positive rate was lower in 3 and 5 year survival cases than that in 3 and 5 year dead cases(P0.01). The Ki67 positive rate had no difference between the patients alive and dead within 3 years, but the 5 years survival rate was lower in Ki67 negative cases(P0.05). ③The over all positive rate of PD4 and Ki67 was 32.41%(47/145) in laryngeal carcinoma tissues and 2.81%(2/71) in non-carcinoma tissues. There was a positive relationship between expression of PD4 and Ki67 (r =0.79). CONCLUSION The high expression of PD4 and Ki67 protein maybe closely related to the occurrence, development and prognosis of laryngeal carcinoma.