Inhibitory effect of resveratrol on human gastric cancer cell line SGC7901 and the possible mechanism

Objective:To investigate the inhibitory effect of Resveratrol(Res)on human gastric cancer cell line SGC7901 and the related mechanism.Methods:Human gastric cancer SGC7901 cells were treated with different concentrations of Res(10,20,40 μg/ml);cells treated with DMSO and culture medium served as controls.The growth inhibition rate of SGC7901 cells was examined by MTT assay;the morphological changes of cells were observed under phase-contrast microscope;caspase-3 activity was assessed by colorimetry;and the cell cycle was detected by flow cytometry(FCM).Results:Res inhibited the growth of SGC7901 cells in a time-and dose-dependent manner,with maximal inhibitory rate being(53.39±5.32)%.After Res treatment the amount of suspended cell increased and the cells shrank,became round and smashed,with particle-like substance found in the cells.The most obvious changes were found 48 h after treatment with 40 μg/ml Res.Besides,Res upregulated the Caspase-3 activity in SGC7901 cells in a time-and concentration-dependent manner.Flow cytometry revealed that Res induced the S phase arrest of SGC7901 cells.Conclusion:Res can obviously inhibit the growth of human gastric cancer SGC7901 cells,possibly through activating Caspase-3,inducing cell apoptosis and influencing cell cycle.