Inhibition of human gastric cancer SGC7901 cells by Resveratrol and its possible mechanism

Objective: To investigate the effects of Resveratrol on the induction of apoptosis of human gastric cancer SGC7901 cells.Methods:Resveratrol(10,20 and 40 μg/mL)affected human gastric cancer SGC7901 cell line,and DMSO and culture liquid were used in control group.The growth inhibition rate of SGC7901 cells was detected by MTT method,the cell shape change was observed by discrepancy microscope and the caspase-3 activity was assessed by colorimetry and the cell cycle was detected by flow cytometry(FCM).Results:Resveratrol inhibited the growth with the dose increase(10～40μg/mL)and the most inhibition rate was(53.39±5.32)%.The suspend cells were increased,and the cell body,shrinked,rounded and smashed,was observed under discrepancy microscope.Resveratrol could increased the caspase-3 activities of SGC7901 cells in a dose-and time-dependent manner.Flow cytometry revealed that Res could decrease reproductive activity by inhibiting cell division.Conclusion:Resveratrol can inhibit human gastric cancer SGC7901 cell and its mechanism might act on Caspase-3,inducing apoptosis because of the increased suspend cell,the shrinked,rounded and smashed cell body under discrepancy microscope,therefore the cycle of tumor cell was affected and tumor cell was inhibited.