Study on Callus Induction and Plantlet Regeneration of Dracaena godsef fiana

Taking the stem segments of Dracaena godseffiana as explant,the callus induction and plantlet regeneration were studied,in order to select the suitable hormone combination in the process of tissue culture.The results indicated that:the optimum medium for callus induction was MS+6-BA 2.0mg/L+TDZ 0.5mg/L+2,4-D 1.0mg/L+sucrose 30g/L+agar 6g/L,induction rate was 88.9%;the optimum medium for adventitious bud differentiation was MS+6-BA 2.0mg/L+NAA 0.5mg/L+sucrose 30g/L+agar 6g/L,induction rate was 59.26%;The optimum medium for adventitious bud proliferating was MS+6-BA 1.0mg/L+NAA 0.8mg/L+GA3 0.2mg/L+sucrose 30g/L+agar 6g/L, proliferation coefficient was 1.91;The optimum medium for rooting was 1/2MS+IBA 1.0mg/L+sucrose 15g/L+agar 6g/L,rooting rate was 81.2%.