Inhibition of mouse lung carcinoma by antisense vascular endothelial growth factor oligonucleotides

OBJECTIVE:To evaluate the inhibitory effection of antisense vascular endothelial growth factor oligonucleotides on lewis lung cancer in a mice model by sa cationic liposomes.METHODS:The 40 nice of the established Lewis lung cancer model were divided into four groups randomly:VEGF-ASODN group, VEGF-SODN group and two control groups.The weight and volume of subcutaneous tumors were measured,and the morphological changes of tumor cells were observed under a microscope.RESULTS:The mean tumor weight of the VEGF-ASODN group was (4.26±1.37) g, that of the VEGF-SODN group was (7.33±1.56) g, and that of the control group was (7.14±1.68) g. The inhibition rates of tumor growth of the VEGF-ASODN group and VEGF-SODN group were 58.2% and 7.4%,respectively. Compared with the control groups, the VEGF-ASODN group showed significance in tumor size and inhibition rates,χ2 were 8.21 and 8.04,P0.01.CONCLUSIONS:The Lewis lung cancer can be inhibited powerfully by antisense VEGF oligonudeotides combined with cationic liposomes. Little side effects are found. The technic of transfection by sa cationic liposomes might be a new method for therapy of cancer.