Effect of VEGF antisense oligonucleotides on growth of Lewis lung cancer

Objective To study the inhibitory effect of VEGF antisense oligonucleotides on growth of Lewis lung cancer in C 57 BL/6 mice.Methods The Lewis lung cancer cells were cultured and implanted subcutaneously into 30 C 57 BL/6 mice,and subsequently the mice were divided into three groups:VEGF ASODN group,VEGF SODN group and the control group.Different treatments were given respectively 24 hours after cell inoculation.The weight and volume of subcutaneous tumors were measured,and the morphological changes of tumor cells were observed under microscope.Results The mean tumor weight of the VEGF ASODN group was ( 4.49 ±0.43)g,that of the VEGF SODN group was (7.73±0.69)g,and that weight of the control group was (7.83±0.78)g.The inhibition rates of tumor growth of the VEGF ASODN group and VEGF SODN group were 42.7% and 5.9%, respectively.Conclusion It is showed that the Lewis lung cancer can be inhibited by the VEGF antisense oligonucleotides inoculated into the center of the tumor in the C 57 BL/6 mice.