2010•Chemistry & BioengineeringRequires access

Study on Quantitative Determination of Dextran by Enzymatic Hydrolysis

Yu Lin

Abstract

With dextran standard solution as the substrate,the quantity of reducing sugar obtained through its hydrolysis by dextranase was determined by DNS spectrophotometry to calculate the content of dextran quantiatively.The results showed that the decomposition rate of dextran by dextranase was 56.0%,and the recovery rate of dextran was(100±5)%,determination deviation of dextran content was no more than ±10% compared with those of Roberts-copper method and Haze-alcohol precipitation method.This study provided a rapid and easy quantitative determination method for dextran.

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Key concepts: Dextranase, Dextran, Chemistry, Hydrolysis, Chromatography, Substrate (aquarium), Precipitation, Quantitative analysis (chemistry)