T. Y. Koh, Boen Tie Khouw
Abstract
A method, based on the liberation of a dye complex from Blue Dextran 2000, is described for the assay of dextranase. The reaction was conducted at 40 °C for 5 min at pH 4.0 in a mixture containing 0.5% Blue Dextran 2000 and the enzyme. The Km for the hydrolysis of Blue Dextran 2000 was estimated at 2.5 × 10−6 M.
OpenAlex reports 21 citations for this work. Citation counts describe recorded attention and do not establish research quality.
A contribution statement is not available in the OpenAlex record.
Method details are not available in the OpenAlex metadata.
Findings are not separately available in the OpenAlex metadata.
Limitations are not available in the OpenAlex metadata.
Application details are not available in the OpenAlex metadata.
A method, based on the liberation of a dye complex from Blue Dextran 2000, is described for the assay of dextranase. The reaction was conducted at 40 °C for 5 min at pH 4.0 in a mixture containing 0.5% Blue Dextran 2000 and the enzyme. The Km for the hydrolysis of Blue Dextran 2000 was estimated at 2.5 × 10−6 M.
Key concepts: Dextranase, Dextran, Hydrolysis, Chemistry, Chromatography, Liberation, Enzyme, Biochemistry