Determination of Five Saponins From Sanqi Capsule by Ultra Fast Liquid Chromatography

Objective To establish an ultra fast liquid chromatography(UFLC) method for determining five saponins in sanqi capsule.Methods Chromatography was performed on an shimadzu C18 column(75 mm×2.0 mm,2.2 μm) at 25 ℃.The gradient elution was acetonitrile-water at a flow rate of 0.3 mL·min-1.The detection wavelength was at 203 nm.Results The linear ranges for notoginsenoside R1,ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and ginsenoside Rd were 9.38-351.62 ng(R2=1),52.11-1 954.12 ng(R2=0.999 9),6.85-152.17 ng(R2=0.999 6),44.99-1 687.25 ng(R2=1),9.96-221.27 ng(R2=1),respectively.The average recoveries were 96.6% for notoginsenoside R1(RSD=0.9%),97.1% for ginsenoside Rg1(RSD=1.4%),101.0% for ginsenoside Re(RSD=1.4%),97.9% for ginsenoside Rb1(RSD=2.4%),98.2% for ginsenoside Rd(RSD=1.8%),respectively(n=6).Conclusion The method is simple,rapid and suitable for determination of five saponins in sanqi capsule.