Determination of Notoginsenoside R_1,Ginsenoside Rg_1,Ginsenoside Re and Ginsenoside Rb_1 in Compound Sanqi Sustained-release Tablets by HPLC

Objective To establish the method of determining effective components in Compound Sanqi Sustained-release Tablets by HPLC.MethodsThe contents of notoginsenoside R1,ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 were simultaneously determined by HPLC conditions as follows:the column was Agilent C18(250 mm×4.6 mm,5μm),the mobile phase was CH3CN-0.05%H3PO4(CH3CN 0~12 min:22%;12~60 min:22%~40%),the flow rate was 1.0 ml·min-1,the detection wavelength was 203 nm.ResultsThe linear range of notoginsenoside R1,ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1,respectively,were 0.373~3.73μg(r= 0.999 7),0.937~9.37 μg(r= 0.999 1),0.144~1.44μg(r=0.999 1),0.893~8.93 μg(r=0.999 0);the average recoveries(n=4) were 99.12%,98.86%,99.25% and 98.63% respectively,and RSD were 1.31%,1.57%,1.75% and 1.68%.ConclusionThe results indicate that the HPLC method is simple,accurate,highly selective and reproducible,thus it can be used for quality control of Compound Sanqi Sustained-release Tablets.