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2007•The Chinese Journal of Modern Applied PharmacyRequires access

Content Determination of Oleanolic Acid in Mufeng Capsules by HPLC

Xiuqin Wang

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Abstract

OBJECTIVE To set up a method for determining oleanolic acid in Mufeng capsules.METHODS HPLC,mobile phase:methanol-0.4% H3PO4 solution(90:10),detection wavelength:210 nm.RESULTS The standard curve was linear in the range of 50.2～351.4 μg·mL-1.The average recovery and RSD% were 98.32% and 1.1%.CONCLUSION This method is accurate,reliable and provide a scientific index for controlling the qualities of Mufeng capsules.

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What this paper is about

OBJECTIVE To set up a method for determining oleanolic acid in Mufeng capsules.METHODS HPLC,mobile phase:methanol-0.4% H3PO4 solution(90:10),detection wavelength:210 nm.RESULTS The standard curve was linear in the range of 50.2～351.4 μg·mL-1.The average recovery and RSD% were 98.32% and 1.1%.CONCLUSION This method is accurate,reliable and provide a scientific index for controlling the qualities of Mufeng capsules.

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Available abstract

OBJECTIVE To set up a method for determining oleanolic acid in Mufeng capsules.METHODS HPLC,mobile phase:methanol-0.4% H3PO4 solution(90:10),detection wavelength:210 nm.RESULTS The standard curve was linear in the range of 50.2～351.4 μg·mL-1.The average recovery and RSD% were 98.32% and 1.1%.CONCLUSION This method is accurate,reliable and provide a scientific index for controlling the qualities of Mufeng capsules.

Key concepts: Oleanolic acid, Chromatography, High-performance liquid chromatography, Content determination, Linear range, Chemistry, Linear relationship, Methanol

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