ScholarLens Back to search
2008•Unpublished venueRequires access

RP-HPLC Determination of Oleanohc Acid in Chaoan capsules

Hongyin Chen, Meng Ju

Open publisher page 0 citations

Abstract

Objective To establish an RP-HPLC method for the determination of oleanolic acid in Chaoan capsules Methods A Kromasil-C18 Column(4.6mm×150mm,5um)was used with the mobile phase being aceto-nitrile-water- phosphate(80:20:0.5),flow rate being 0.8 ml.min-1,determinating wavelength being 208 nm,the column temperature being 25℃,Results The calibration curve was linear in the range of 1.124～5.620μg for oleanolic acid(r=0.9999),and the regression equation being Y=381943×X+1651,The average recovery was 100.79%,with RSD of 1.10%.Gonclusion The method is simple,accurate and reproducible for quality control of Chaoan capsules.

About this research paper

What this paper is about

Objective To establish an RP-HPLC method for the determination of oleanolic acid in Chaoan capsules Methods A Kromasil-C18 Column(4.6mm×150mm,5um)was used with the mobile phase being aceto-nitrile-water- phosphate(80:20:0.5),flow rate being 0.8 ml.min-1,determinating wavelength being 208 nm,the column temperature being 25℃,Results The calibration curve was linear in the range of 1.124～5.620μg for oleanolic acid(r=0.9999),and the regression equation being Y=381943×X+1651,The average recovery was 100.79%,with RSD of 1.10%.Gonclusion The method is simple,accurate and reproducible for quality control of Chaoan capsules.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To establish an RP-HPLC method for the determination of oleanolic acid in Chaoan capsules Methods A Kromasil-C18 Column(4.6mm×150mm,5um)was used with the mobile phase being aceto-nitrile-water- phosphate(80:20:0.5),flow rate being 0.8 ml.min-1,determinating wavelength being 208 nm,the column temperature being 25℃,Results The calibration curve was linear in the range of 1.124～5.620μg for oleanolic acid(r=0.9999),and the regression equation being Y=381943×X+1651,The average recovery was 100.79%,with RSD of 1.10%.Gonclusion The method is simple,accurate and reproducible for quality control of Chaoan capsules.

Key concepts: Chromatography, Oleanolic acid, Calibration curve, High-performance liquid chromatography, Chemistry, Linear range, Detection limit, Medicine

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
RP-HPLC Determination of Oleanohc Acid in Chaoan capsules — Research Paper | ScholarLens