Quantitative Determinnation of Chlorogenic Acid and Baicalin in Qingrejiedu oral liquid by HPLC

Objective To develop an RP - HPLC method for the determination of chlorogenic acid and baicalin in Qingrejiedu oral liquid. Methods The chromatographic column was Kromasil C18(150×4.6 mm,5μm),The mobile phase was CH_3OH-0.4%Phosphoric acid.The flow rate was set at 1.0ml/min.The detection wavelength was at 327 nm.Chlorogenic acid and baicalin were separated by HPLC with grade elution.Results The linearity ranges of chlorogenic acid and baicalin were 3.97 -99.2μg/mL and 16.24 ~406.0μg/mL respectively, the correlations coefficients were 0.999 4 and 0.999 5,the average recoveries of adding sample were 99.92%and 100.31%, the detection limits were 0.07μg/mL and 0.12μg/mL,the RSD(re =5) of measurement precisions test were 1.03%and 1.21%,the RSD (n=5) of reproducibility between tests were 0.23%and 0.78%,the RSD(n=5) of stability test were 1.99%and 1.09%.The content of chlorogenic acid in compound Qingrejiedu oral liquid was 0.126 -0.238 mg/mL,and that of baicalin was 1.157～3.079 mg/mL.Conclusion The method is simple,fast,accurate,sensitive and reproducible.It is fit for the quality control of compound Qingrejiedu oral liquid.