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Application of Pulsed Field Gel Electrophoresis in Molecular Typing of Salmonella Typhimurium

Ji Hui

Abstract

To study the application of Salmonella Typhimurium typing by the technique of pulsed field gel electrophoresis (PFGE). The genomic DNA in different Salmonella strains was isolated and separated on PFGE after digesting with Xba I.The electrophoresis patterns were analyzed using the Freeview software. DNA isolated from 13 strains was classified into 10 different subtypes according to the PFGE patterns. PFGE technique can be used for molecular typing of Salmonella subtypes and typing result is of stability.

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Key concepts: Pulsed-field gel electrophoresis, Typing, Salmonella, Gel electrophoresis, Biology, Microbiology, Genetics, Bacteria