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2005•The Chinese Journal of Modern Applied PharmacyRequires access

Determination of puerarin in Ganmao Qingre grains by RP-HPLC

Zhengguo Li

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Abstract

OBJECTIVE To determine puerarin in Ganmao Qingre grains.METHODS C_ 18 column,detection wavelength 250nm and the mobile phase of methanol-1.4%acetic acid (22∶78) were used in RP-HPLC.RESULTS The recovery of the method was 99.59% (RSD=2.3%, n =5).CONCLUSION This method is simple, accurate and can be used for quality control of Ganmao Qingre grains.

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OBJECTIVE To determine puerarin in Ganmao Qingre grains.METHODS C_ 18 column,detection wavelength 250nm and the mobile phase of methanol-1.4%acetic acid (22∶78) were used in RP-HPLC.RESULTS The recovery of the method was 99.59% (RSD=2.3%, n =5).CONCLUSION This method is simple, accurate and can be used for quality control of Ganmao Qingre grains.

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Available abstract

OBJECTIVE To determine puerarin in Ganmao Qingre grains.METHODS C_ 18 column,detection wavelength 250nm and the mobile phase of methanol-1.4%acetic acid (22∶78) were used in RP-HPLC.RESULTS The recovery of the method was 99.59% (RSD=2.3%, n =5).CONCLUSION This method is simple, accurate and can be used for quality control of Ganmao Qingre grains.

Key concepts: Puerarin, Chromatography, High-performance liquid chromatography, Chemistry, Medicine, Pathology, Alternative medicine

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