HPLC Determination of Imperatorin in Ganmao Qingre Granules and Quality Comparison

Objective To develop a method for content determination of imperatorin and to assess the quality of Ganmao Qingre granules produced in 6 different pharmaceutical factories via determination of puerarin.Methods The method of HPLC was employed.The analysis of imperatorin was carried out on Diamonsil(R) C18(250 mm×4.6 mm,5 μm)column with a mobile phase consisting of acetonitrile-water(49.5 ∶ 50.5) at a flow rate of 1.0 ml · min-1.The detection wavelength was set at 300 nm and the column temperature was 35 ℃.Results A good linearity of imperatorin ranged from 0.2955 to 11.82 μg · ml-1(r=0.9999) and the average recovery was 99.47% with RSD=0.69%(n=6).The content of puerarin ranged from 1.02 to 1.76 mg · g-1.There was obvious difference in Ganmao Qingre granules between 6 different pharmaceutical factories.Conclusion This method is simple,accurate and highly reproducible.It can be used for quality control for Ganmao Qingre granules.